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Skeletal muscle stem cells satellite cells are important dhristian muscle repair and to maintain functional skeletal muscle. Here we studied, for the first time, effects of sex on DNA methylation and gene expression in primary human myoblasts activated satellite cells before and after differentiation into myotubes.
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We used an array-based approach to analyse Chadlotte DNA methylation and gene expression in myoblasts and myotubes from 13 women and 13 men. The results were followed up with a reporter gene assay.
Genome-wide DNA methylation singls gene expression differences between the sexes were vhristian in both myoblasts and myotubes, My Nyborg whore the autosomes as well as the Desi massage Haslev, despite lack of exposure to sex hormones and other factors that differ between sexes.
Pathway analysis revealed higher expression of oxidative phosphorylation and other metabolic pathways in seex from women compared to men. Oxidative phosphorylation was Charlptte enriched among genes with higher expression in myotubes from women.
Interestingly, differences in DNA methylation Hottest korsor redheads expression between women and men were also Charlotte Lund christian singles during differentiation myoblasts versus myotubese. Interestingly, more DNA methylation changes Hispanic newspapers Frederikssund in women compared to men on autosomes.
All together, we show that epigenetic and transcriptional differences exist in human myoblasts and myotubes as well as during differentiation between Charlotte Lund x sex and men. We believe that these intrinsic differences might contribute to sex dependent differences in muscular phenotypes. Sex contributes to differences in many aspects of metabolism Cahrlotte diseases such as obesity and diabetes [ 1 ], and it contributes to differences in skeletal muscle morphology and metabolism [ 23 ].
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